User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/18
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PCR of B0015
The point of this PCR is get double terminator and insert it into pSB1T3 backbone.
PCR of pSB1T3
All 2X. Two PCRs.
Gel PCR of pSB1T3 backbone
As expected :) Both amplified.
Purification of PCR product
Both were purified with Roche´s Kit for PCR purification
I performed restriction with EcoRI and PstI.