User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/29

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM 2011 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objectives

  • Grow in solid medium BBa_B0015 and liquid for extraction.
  • See gel from extractions of pUX19 cut with SmaI.
  • Digestion of pSB1AK3/BBa_B0015 with XbaI to see size.

Gel of SmaI restriction

I expect 6.2Kbp~

SmaIcut.pUX19.27.jul.2011.jpg

Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.

Extraction

Extraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep.

PartBBa B0015.KAres.29jul2011.JPG

I just need it to see concentration for digestion.

Digestion

Reactives μL
XbaI 1
Buffer4 NEB10X 2
BSA10X 2
DNA of pSB1AK3/BBa_B0015 12
H2O 3
Total 20

Over night reaction.