User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/20

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PCR‡ -R -F -DNA -Oligos 5μL DNA 10μL DNA
Forward 2 0 2 0 2 2
Reverse 0 2 2 0 2 2
dNTPs .4 .4 .4 .4 .4 .4
Buffer 4 4 4 4 4 4
Phusion .2 .2 .2 .2 .2 .2
DNA 10 10 0 10 5 10
H2O 3.4 3.4 11.4 5.4 6.4 1.4
    • ‡ μL Units
    • Phusion protocol. H2O Full up 20μL