User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/10/07

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October 7th, 2010

1. Extract plasmid from the following strains:

  • pSB1A2 + J23101 + ΔRBS + GFP E004
  • pSB3K3 + J23101
  • pSB4A5 + MinBP
  • pSB1C3


2. Make the following restrictions:

  • pSB1C3 EcoRI-PstI restriction
  • EcoRI-PstI double restriction methods:
DNA -> 5 ul
Buffer 3 -> 2 ul (10% of total volume)
BSA -> 1 ul
EcoRI -> 1 ul
PstI -> 1 ul
HPLC -> 10 ul (to complete total volume of 20ul)
Incubate at 37º C for 4 hrs.