User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/08/08

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August 8th, 2010

1. Incubate BBa_I20260 (pSB3K3 + J23101 promoter + GFP E0040) strain in liquid LB medium at 37ºC overnight to perform plasmid extraction.

  • The plasmid will be used to make PCR and change RBS because we have one construction with this plasmid + Blue Promoter + GFP E0240, and we want to have both GFP’s under the same RBS, so we can characterize better the promoters without noise caused if we used different Ribosome Binding Sites.