User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/08/04

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August 4th, 2010

1. Run gel to verify PCR made on August 3rd.

Test L2-L4 4Aug2010.JPG

Lanes: 1) Green ladder; 2) L2 Preff FWD-Suff REV; 3) L4 Preff FWD-Suff REV; 4) L2 BluePromoter FWD-BluePromoter REV; 5) L4 BluePromoter FWD-BluePromoter REV; 6) Positive control using L1 with Preff FWD-Suff REV.

It seems constructions are OK, but I’ll check that size of the products correspond to the expected size of Blue Promoter + GFP.

2. Incubate in liquid BOSS medium MJ11 strain.