User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/07/29

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July 29th, 2010

1. Plate in solid LB medium TrpR mutant strains grown on July 28th.

2. Design reverse primer to amplify BBa_I20260: J23101 promoter + GFP BBa_E0040, the primer will anneal in the J23101-promoter region and will have a SpeI restriction site.

>Primer J23101 + SpeI REV

actagt gta gctagcataatacctaggactgagctagc

Primer has 6bp of SpeI restriction site, 3bp of separation between restriction site and promoter, and 29bp of J23101.