User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/07/08

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July 8th, 2010

1. Inactivate enzyme from restrictions made on June 7th. Incubate for 20 min at 80ºC.


2. Run gel to verify extracted plasmids, PstI and EcoRI-PstI restrictions made on June 7th.

Plasmids and restrictions 8Jul2010-1.JPG

Lanes: 1) Ladder; 2) Plasmid L1; 3) Plasmid L2; 4) Plasmid L4, 5) Plasmid L5; 6) Plasmid L6; 7) p18; 8) p19; 9) p30; 10) L1 PstI; 11) L2 PstI; 12) L4 PstI; 13) L5 PstI; 14) L6 PstI; 15) Ladder.

Plasmids and restrictions 8Jul2010-2.JPG

Lanes: 1) Ladder; 2) p18 PstI; 3) p19 PstI; 4) p30 PstI; 5) L1 EcoRI-PstI; 6) L2 EcoRI-PstI; 7) L4 EcoRI-PstI; 8) L5 EcoRI-PstI; 9) L6 EcoRI-PstI; 10) p18 EcoRI-PstI; 11) p19 EcoRI-PstI; 12) p30 EcoRI-PstI; 13) Ladder.


3. Incubate cells on 5 ml of liquid LB with antibiotic (0.5ul:1ml)

  • L1: p30-MinBP + GFP E0240
  • L2: p30-MinBP + GFP 74 min
  • Standard promoter J23101 + GFP E024
  • GFP reporter Eo240 without promoter