User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/07/06

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July 6th, 2010

1. Replate transformations from July 3rd.

  • L1: p30-MinBP + GFP E0240 (6 colonies)
  • L4: Backbone pSB3K3 + BP + GFP BBa_K145015 (74 min) (6 colonies, just colonies 1-3 appeared on sunday morning)
  • L5: Plasmid 18 + GFP BBa_K145015 (3 colonies, all of them appeared on sunday morning)
  • p19 (Ctrl +)

2. I also replated transformation Backbone pSB3K3 + Blue Promoter + GFP E0240, this transformation was done on June 29th, I had already replated it on July 2nd but nothing grew, let’s see what happens now.

3. Prepare culture dishes with Kan, procedure is described on April 20th.