User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/05/07

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May 7th, 2010

1. Transformation with BBa_K145015 from may 6th was not successful because I used only 3ul of plasmid, today I`ll remake transformation with 100ul of competent cells and 6 ul of plasmid, positive control is plasmid 19 (BBa_I51020), 3ul.

2. Genomic DNA extraction from E. coli K12

  • DNA extraction was made following the Amersham Biosciences GenomicPrep Cells and tissue DNA Isolation Kit for 1ml of gram-negative bacteria. Tubes were marked DNA K12 1 and DNA K12 2, in tube one I made a mistake in step 5.3 because I didn’t mix the cell lysis solution with the cells, so I repeated the extraction in tube 2.