User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/30
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Wednesday 30th June 2010
I checked the disgestions on an agarose gel to confirm the existence of the ligation products expected, but the gel looks the same!! The ligations aren’t the ones we expected, so I’ll have to do it all over again.
1º and 8º lanes are the DNA ladders
I also extracted plasmids 18, 19 and 30, by growing them first in 4 ml of liquid LB with 4ul of the respective antibiotic, and the extract the plasmid with the High Pure Plasmid Isolation Kit from Roche.
Plasmid 19 was also replated in a petri box of LB and ampicillin.
1º lane is the ladder