User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/16

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Wednesday 16 June 2010

I performed a double restriction of the cI inverter plasmid with SpeI and PstI as follows:

Reactive...........1x [ul]
DNA................10
BSA..................1
Buffer2.............3
H2O................13
SpeI.................1.5
PstI..................1.5
Total................30
The digestion was left in the incubator at 37º C for 4 hours.