User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/16

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Wednesday 16 June 2010 I performed a double restriction of the cI inverter plasmid with SpeI and PstI as follows: Reactive...........1x [ul] DNA................10 BSA..................1 Buffer2.............3 H2O................13 SpeI.................1.5 PstI..................1.5 Total................30 The digestion was left in the incubator at 37º C for 4 hours.