User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/09

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Wednesday 9th June 2010

I performed a double restriction on the plasmid with the cI inverter using EcoRI and PST1. Then I checked it on an agarose gel but there seemed not to be a band of 1kb from the restricted fragment.

IMG 0204.JPG

1º lane is the 1kb DNA ladder
2º lane is the plasmid
3º is the double restriction with PSTI and EcoRI

There should be a 1kb band but there isn’t.