User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/05/06
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Thursday 6th May 2010
I cultured the transformations in 4ml of liquid LB with 4 ul of ampicillin each one, then extracted plasmid with the Rocher Kit and made a double restriction using EcoR1 and PST1 as follows:
Reactive....1x [ul].......5x [ul]
Then I placed the restrictions to incubate overnight.