User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/05/06

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Thursday 6th May 2010

I cultured the transformations in 4ml of liquid LB with 4 ul of ampicillin each one, then extracted plasmid with the Rocher Kit and made a double restriction using EcoR1 and PST1 as follows:

Reactive....1x [ul].......5x [ul]
DNA..........5...............---
BSAa.........1...............5
Buffer2.....2...............10
H2O.........10..............50
EcoR1.......1...............5
PST1.........1...............5
Total........20.............100

Then I placed the restrictions to incubate overnight.
I also saved the plasmids extracted in the freezer, everything is very well labeled.