User:Jerome Bonnet/Notebook/ Writing genetic memory with integrases

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Abstract

I will use site phage integrases to control switching between 2 DNA states. These enzymes catalyze unidirectional recombination events between two different specific DNA sequences, named attachment or att sites, leading to the integration of the phage genome into the bacterial host genome (Groth and Calos, 2005). The reaction is reversed by association of a specific excisionase subunit (figure 1A). Interestingly, Integrase/Excisionases pairs can control the inversion of a target sequence flanked by two different att sites located in the same construct (figure 1B; mizuuchi et al., 1980; Lorbach et al, 1999), allowing for full control of reaction reversibility (Groth and Calos, 2005, Grindley et al., 2006). Sequence inversion can in turn be used to control gene expression( see also Ham et al., 2006, 2008, for a system based on invertases). It is a promising system to store multiple states within DNA sequence with full control on memory location and state switching. Many thanks to Alfonso Farrugio and Michele Calos for help on this topic (Calos lab).

  • Figure 1A: Natural integration/excisions reactions



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  • Figure 1B: proposed mechanism for writing 1 bit of memory

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Experimental design

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