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Objective
Add a new syringe to the pump in order to supply glutaraldehyde to the PVA mixture in order to crosslink the PVA polymer beads.
Description
- Glutaraldehyde must be added to PVA mixture in 1:2 ratio
- Syringe method is used to piece tygon tubing
- Tygon tubing with carrier fluid must be long enough such that with a certain flow-rate the PVA and glutaraldehyde may react for 5 min
Data
- Rates for syringe pumps
- Red pump: 0.05 mL/min
- Grey pump: 0.1 mL/min
- Final Length of tygon tubing: 50.5 cm
- Diameter of tygon tubing: Calculations for correct length and reaction time of 5 min
- radius of tubing= 0.0397 cm
- V = pi*r^2*L
- (0.05 mL/min*5 min) = 3.14 * 0.00158 cm^2 * L
- 0.25 mL / (3.14 * 0.00158 cm^2) = L
- L = 50.5 cm
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481 Experimental Chemistry
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