User:Jeffrey Kim/Notebook/High-throughput metagenomic profiling of secondary metabolites

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">03/18/2009,01/26/2010</div><div style="display:none;" id="page">User:Jeffrey Kim/Notebook/High-throughput metagenomic profiling of secondary metabolites</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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High-throughput metagenomic profiling of secondary metabolites

  • Touchdown Phusion protocol

98C 30s


98C 10s 72C 30s 72C 30s


(7x TD -2C/cycle)

98C 10s 58C 30s 72C 30s (29x)


72C 4 minutes

Standard phusion

98C 30s


98C 10s ann 30s 72C 30s


72C 5 minutes

(AD = 72C annealing),

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