User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/26

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  • Captured YAC/BAC from ZB118 is a pain in the #$$ to grow up and isolate in sufficient quantity using glass-bead smash and grab prep. The construct may be too large and fragile to handle like this so I ordered some zymolyase and a proper resin isolation kit from zymoprep.
  • Electroporated DNA is simply too low in quality/concentration to be of any colonies on tet selection.