User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/22

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TAR

  • pBC-436 dd XbaI SpeI to release 436 fragment
  • pLLX13 digested with AvrII to insert fragment
  • Ligation setup and electroporated. Outgrown, plated on tet 5 apra 50 plates.