User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/14

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TAR cloning

  • Recombined plasmids selected on AMP/TET (100/5ug/ml) grown overnight at 37 in EC100's look good. No background at all and ~150 colonies on recombined sample.
  • 10 clones selected for growth in culture (selection) at 37C with shaking. Will analyze plasmids to see if the recombination worked efficiently.