User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2008/09/28

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  • Recombination experiments started with pOJ436 APRamycin cassette amplification off of PiJ778. Yield was low due to bad enzyme but proceeded with recombination.
  • Competent cell prep from 10ml SOB with 100mM induction with L-arabinose a-la DK (30C 3hr ~0.5-OD, 30min spike with 100mM arabinose prior to comp cell prep)
  • Recombination after electroporation for 1hr at 37C. entire reaction plated on amp apra plates (50/50) and grown at 37 ON