User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/04/01

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Entry title Insert content here... Objective Run UV-Vis and AA on cooled 30:1 lysozyme-AuNP samples from 3/25/14. Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14. Procedure Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14 1. Add 2.25 mL of each variable to 4.5 mL of sample. The variables used were: 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) 2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C. Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature Atomic Absorption Preparation Creating the Gold Stock Solutions Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au. Atomic Absorption Samples Solutions with the following Au:lysozyme ratio at room temperature were run on the AA: 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine) 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine) 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine) Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube. Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created. Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate. Figures