Entry title
Objective
- Run UV-Vis and AA on cooled 30:1 lysozyme-AuNP samples from 3/25/14.
- Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14.
Procedure
Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14
1. Add 2.25 mL of each variable to 4.5 mL of sample.
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- 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
- 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
- 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.
Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature
Atomic Absorption Preparation
Creating the Gold Stock Solutions
- Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
- Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
- Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
- Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
- Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.
Atomic Absorption Samples
Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:
- 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
- 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
- 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)
Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples
- Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube.
- Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created.
- Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate.
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