User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/16

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We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol


Stock Solution

  1. Buffer
    1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
    1. Dissolve 12.9mg luminol in 300uL of DMSO
    2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
    1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
    2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM

We run UV-VIS sample of Citrate and HRP with AUNP, and after analyzed the data correcting the absorbance we observed the following graph.

For citrate the peak was 523 nm

For HRP the peak was 526nm

"Figure 1. Absorbance of Citrate-AUNP and HRP-AUNP"

Absorbance of Citrate-AUNP and HRP-AUNP.png

The we run the reaction on kinetics, but no activation of the protein was observed as it can be seen in the following figure.

"Figure 2. HRP-AUNP luminol |500px "

HRP AUNP luminol.png

We don't know why no activation of the protein happened in the nanoparticles of gold.