User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/09
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We are running the same procedure as yesterday only with an inhibitor, EHNA.
The reaction samples will contain roughly 1nM EHNA
After we collected the data, we worked up the data and we found the concentration of inosine and adenosine, based on beer's law, and the peaks 260 and 250, from which we know epsilon, b and the absorbance. After putting the files and working it up, we obtained the graph of Adenosine to Inosine.
"Figure 1. Adenosine to Inosine"
"Table 1. System of equation to calculate concentration of Adenosine"
"Table 2. System of equation to calculate concentration of Inosine"
Then after this, we set up a system of equations to find out the concentration at different points during the time the reaction took, and sketched a graph.
"Figure 2. Change in concentrations from Adenosine to Inosine"