User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/02

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Procedure

  1. Chemiluminescence of luminol oxidation reaction initiated by stopped flow mixer
    1. Add HRP/Luminol stock solution to stopped flow mixer
    2. Add H2O2 stock solution to stopped flow mixer
    3. equilibrate mixer tubes with sample.
    4. Initiate Mixing
    5. Measure light produced as result of reaction, integrated over a specific time range
    6. Integrate area under the curve

While running the samples, it did not work due to errors from machine, thus we were not able to get any results from the machine.

Today, we worked up the data of Trisha's group due to the fact that no other data worked. The peaked of the concentration for this figure is 390 nm

"Figure 1. Decrease in concentration of luminol during the oxidation of luminol by HRP

Decrease in concentration luminol by oxidation JAvier Vinals.png

We worked up the data of Trisha's group due to the fact that no other data worked. The peaked of the concentration for this figure is 425 nm

"Figure 2. Increase in concentration of luminol during the oxidation of luminol by HRP"

Increase in concentration of luminol during the oxidation of luminol by HRP.png