User:Jamie Nunziata/Notebook/Protease Research/2015/09/16
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To make more AuNP solutions to replace any that didn't work from the previous lab day and to practice making buffer solutions.
Procedure and Results
The full set of procedure can be found inthe notebook entry Dr. Hartings left for today.
We first began correcting our failed AuNP solutions. Our 5 mL samples did make nanoparticles but 16 1mL samples were remade.
To make the buffers, 500mL of 10mM Tris (pH 9) and 500mM of 10mM phosphate buffer (pH 7) were made using the calculated values from location for Tris and from location for the phosphate buffer. We plugged in our desired concentration and volume into the calculator and it gave us the following protocol
The pH probe was then calibrated to the pH 4, 7, and 10 standard solutions. When we measured out buffers, they were not quite accurate. After adding acid to our Tris buffer, we managed to get it to 9.04. Our phosphate buffer was at 7.4, so HCl was also added to bring it down to 7.
Fortunately, upon future observation, 8 our of AuNP samples were synthesized successfully.