User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/02/22

Biomaterials Design Lab - Spring 2016

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Objective Re-create lysozyme nanoparticles Protocol The creation of nanoparticles on 02/16/2016 failed because used a smaller lysozyme to gold ratio and made fibers instead, which we will now incorporate into a film. This time we are using a larger ratio in hopes to make lysozyme nanoparticles. Lysozyme Nanoparticle Setup 100mL stock solution was made using the following steps: 3.35mg of lysozyme was added to 10mL of water in a volumetric flask (2.47M). 5 mL of the solution was then transferred to a 150mL beaker. 0.84mL of 1% by weight HAuCl4 stock solution was added (made on 01/27/2016) 94.16mL of water was added, bringing the final solution volume up to 100mL. The solution was then covered with foil and placed in the oven for 4 hours at 80 degrees celsius. Results We made fibers instead of nanoparticles. Again. Next time we will double the original concentration of lysozyme to hopefully make nanoparticles.