User:James L Hartley

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Contact Info

James L Hartley (an artistic interpretation)
  • James L Hartley
  • National Cancer Institute
  • Protein Expression Laboratory
  • PO Box B, Building 327 Room 4
  • Frederick, MD 21702 USA
  • hartleyjames@mail.nih.gov

I learned about OpenWetWare from Google search for origin of E. coli RR1 strain, and I've joined because I feel like I can contribute to some content. >30 years experience in DNA cloning and protein expression.

Education

  • 1975 Ph.D. Colorado State University
  • 1981 - 2001 Research scientist, Bethesda Research Laboratories / Life Technologies, Inc. / Invitrogen
  • 2001 - 2011 Director, Protein Expression Laboratory, NCI Frederick [Contractor]

Research interests

  1. DNA technologies
  2. Protein technologies
  3. High-level themes in biology



Publications

1: Gillette WK, Esposito D, Taylor TE, Hopkins RF, Bagni RK, Hartley JL. Purify First: rapid expression and purification of proteins from XMRV. Protein Expr Purif. 2011 Apr;76(2):238-47. Epub 2010 Dec 10. PubMed PMID: 21146612.


2: Ganatra MB, Vainauskas S, Hong JM, Taylor TE, Denson JP, Esposito D, Read JD, Schmeisser H, Zoon KC, Hartley JL, Taron CH. A set of aspartyl protease-deficient strains for improved expression of heterologous proteins in Kluyveromyces lactis. FEMS Yeast Res. 2011 Mar;11(2):168-78. doi: 10.1111/j.1567-1364.2010.00703.x. Epub 2010 Dec 17. PubMed PMID: 21166768; PubMed Central PMCID: PMC3041862.


3: Kaczmarczyk SJ, Sitaraman K, Hill T, Hartley JL, Chatterjee DK. Tus, an E. coli protein, contains mammalian nuclear targeting and exporting signals. PLoS One. 2010 Jan 26;5(1):e8889. PubMed PMID: 20126275; PubMed Central PMCID: PMC2811178.


4: Hartley JL, Salehi-Ashtiani K, Hill DE. Proteome expression moves in vitro: resources and tools for harnessing the human proteome. Nat Methods. 2008 Dec;5(12):1001-2. PubMed PMID: 19034266.


5: Warfield KL, Posten NA, Swenson DL, Olinger GG, Esposito D, Gillette WK, Hopkins RF, Costantino J, Panchal RG, Hartley JL, Aman MJ, Bavari S. Filovirus-like particles produced in insect cells: immunogenicity and protection in rodents. J Infect Dis. 2007 Nov 15;196 Suppl 2:S421-9. PubMed PMID: 17940979.


6: Baba M, Hong SB, Sharma N, Warren MB, Nickerson ML, Iwamatsu A, Esposito D, Gillette WK, Hopkins RF 3rd, Hartley JL, Furihata M, Oishi S, Zhen W, Burke TR Jr, Linehan WM, Schmidt LS, Zbar B. Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling. Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15552-7. Epub 2006 Oct 6. PubMed PMID: 17028174; PubMed Central PMCID: PMC1592464.


7: Hartley JL. Cloning technologies for protein expression and purification. Curr Opin Biotechnol. 2006 Aug;17(4):359-66. Epub 2006 Jul 12. Review. PubMed PMID: 16839756.


8: Giomarelli B, Schumacher KM, Taylor TE, Sowder RC 2nd, Hartley JL, McMahon JB, Mori T. Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture. Protein Expr Purif. 2006 May;47(1):194-202. Epub 2005 Nov 7. PubMed PMID: 16300962.


9: Gillette WK, Esposito D, Frank PH, Zhou M, Yu LR, Jozwik C, Zhang X, McGowan B, Jacobowitz DM, Pollard HB, Hao T, Hill DE, Vidal M, Conrads TP, Veenstra TD, Hartley JL. Pooled ORF expression technology (POET): using proteomics to screen pools of open reading frames for protein expression. Mol Cell Proteomics. 2005 Nov;4(11):1647-52. Epub 2005 Aug 19. PubMed PMID: 16113400.


10: Esposito D, Gillette WK, Miller DA, Taylor TE, Frank PH, Hu R, Bekisz J, Hernandez J, Cregg JM, Zoon KC, Hartley JL. Gateway cloning is compatible with protein secretion from Pichia pastoris. Protein Expr Purif. 2005 Apr;40(2):424-8. PubMed PMID: 15766886.


11: Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, Hill DE, Vidal M. Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 2004 Oct;14(10B):2128-35. PubMed PMID: 15489335; PubMed Central PMCID: PMC528929.


12: Cheo DL, Titus SA, Byrd DR, Hartley JL, Temple GF, Brasch MA. Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones. Genome Res. 2004 Oct;14(10B):2111-20. PubMed PMID: 15489333; PubMed Central PMCID: PMC528927.


13: Hill DE, Brasch MA, del Campo AA, Doucette-Stamm L, Garrels JI, Glaven J, Hartley JL, Hudson JR Jr, Moore T, Vidal M. Academia-industry collaboration: an integral element for building "omic" resources. Genome Res. 2004 Oct;14(10B):2010-4. Review. PubMed PMID: 15489319.


14: Brasch MA, Hartley JL, Vidal M. ORFeome cloning and systems biology: standardized mass production of the parts from the parts-list. Genome Res. 2004 Oct;14(10B):2001-9. Review. PubMed PMID: 15489318.


15: Sitaraman K, Esposito D, Klarmann G, Le Grice SF, Hartley JL, Chatterjee DK. A novel cell-free protein synthesis system. J Biotechnol. 2004 Jun 10;110(3):257-63. PubMed PMID: 15163516.


16: Esposito D, Gillette WK, Hartley JL. Blocking oligonucleotides improve sequencing through inverted repeats. Biotechniques. 2003 Nov;35(5):914-6, 918, 920. PubMed PMID: 14628662.


17: Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, Moore T, Hudson JR Jr, Hartley JL, Brasch MA, Vandenhaute J, Boulton S, Endress GA, Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek J, Snyder M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal M. C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet. 2003 May;34(1):35-41. PubMed PMID: 12679813.


18: Hartley JL. Use of the gateway system for protein expression in multiple hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID: 18429245.


19: Shevchenko Y, Bouffard GG, Butterfield YS, Blakesley RW, Hartley JL, Young AC, Marra MA, Jones SJ, Touchman JW, Green ED. Systematic sequencing of cDNA clones using the transposon Tn5. Nucleic Acids Res. 2002 Jun 1;30(11):2469-77. PubMed PMID: 12034835; PubMed Central PMCID: PMC117195.


20: Reboul J, Vaglio P, Tzellas N, Thierry-Mieg N, Moore T, Jackson C, Shin-i T, Kohara Y, Thierry-Mieg D, Thierry-Mieg J, Lee H, Hitti J, Doucette-Stamm L, Hartley JL, Temple GF, Brasch MA, Vandenhaute J, Lamesch PE, Hill DE, Vidal M. Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nat Genet. 2001 Mar;27(3):332-6. PubMed PMID: 11242119.


21: Hartley JL, Temple GF, Brasch MA. DNA cloning using in vitro site-specific recombination. Genome Res. 2000 Nov;10(11):1788-95. PubMed PMID: 11076863; PubMed Central PMCID: PMC310948.


22: Walhout AJ, Sordella R, Lu X, Hartley JL, Temple GF, Brasch MA, Thierry-Mieg N, Vidal M. Protein interaction mapping in C. elegans using proteins involved in vulval development. Science. 2000 Jan 7;287(5450):116-22. PubMed PMID: 10615043.


23: Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 2000;328:575-92. PubMed PMID: 11075367.


24: Hartley JL, Rashtchian A. Dealing with contamination: enzymatic control of carryover contamination in PCR. PCR Methods Appl. 1993 Oct;3(2):S10-4. PubMed PMID: 8268783.


25: Thornton CG, Hartley JL, Rashtchian A. Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling. Biotechniques. 1992 Aug;13(2):180-4. PubMed PMID: 1389144.


26: Longo MC, Berninger MS, Hartley JL. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990 Sep 1;93(1):125-8. PubMed PMID: 2227421.


27: Hartley JL, Berninger M, Jessee JA, Bloom FR, Temple GF. Bioassay for specific DNA sequences using a non-radioactive probe. Gene. 1986;49(3):295-302. PubMed PMID: 3569918.


28: Hartley JL, Donelson JE. Generation of overlapping labeled DNA fragments for DNA sequencing: a mercury-thiol affinity system. Methods Enzymol. 1983;100:116-30. PubMed PMID: 6312253.


29: Hartley JL, Chen KK, Donelson JE. A mercury-thiol affinity system for rapid generation of overlapping labeled DNA fragments for DNA sequencing. Nucleic Acids Res. 1982 Jul 10;10(13):4009-25. PubMed PMID: 7050914; PubMed Central PMCID: PMC320774.


30: Beale EG, Hartley JL, Granner DK. N6,O2'-dibutyryl cycle AMP and glucose regulate the amount of messenger RNA coding for hepatic phosphoenolpyruvate carboxykinase (GTP). J Biol Chem. 1982 Feb 25;257(4):2022-8. PubMed PMID: 6276405.


31: Hartley JL, Gregori TJ. Cloning multiple copies of a DNA segment. Gene. 1981 May;13(4):347-53. PubMed PMID: 6266922.


32: Walder RY, Hartley JL, Donelson JE, Walder JA. Cloning and expression of the Pst I restriction-modification system in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1503-7. PubMed PMID: 6262807; PubMed Central PMCID: PMC319159.


33: Walder RY, Hartley JL, Donelson JE, Walder JA. Cloning of the Pst I restriction-modification system. Gene Amplif Anal. 1981;1:217-27. Review. PubMed PMID: 6765641.


34: Rosen ED, Hartley JL, Matz K, Nichols BP, Young KM, Donelson JE, Gussin GN. DNA sequence analysis of prm-mutations of coliphage lambda. Gene. 1980 Nov;11(3-4):197-205. PubMed PMID: 6452305.


35: Hartley JL, Donelson JE. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860-5. PubMed PMID: 6251374.


36: Gubbins EJ, Maurer RA, Hartley JL, Donelson JE. Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin. Nucleic Acids Res. 1979 Mar;6(3):915-30. PubMed PMID: 375200; PubMed Central PMCID: PMC327742.


37: Hartley JL, Soffer RL. On the oligosaccharide moiety of angiotensin-converting enzyme. Biochem Biophys Res Commun. 1978 Aug 29;83(4):1545-52. PubMed PMID: 212068.


38: Conroy JM, Hartley JL, Soffer RL. Canine pulmonary angiotensin-converting enzyme. Physicochemical, catalytic and immunological properties. Biochim Biophys Acta. 1978 Jun 9;524(2):403-12. PubMed PMID: 208622.


39: Das M, Hartley JL, Soffers RL. Serum angiotensin-converting enzyme. Isolation and relationship to the pulmonary enzyme. J Biol Chem. 1977 Feb 25;252(4):1316-9. PubMed PMID: 190228.


40: Hartley JL, Adams GA, Tornabene TG. Chemical and physical properties of lipopolysaccharide of Yersinia pestis. J Bacteriol. 1974 Jun;118(3):848-54. PubMed PMID: 4829929; PubMed Central PMCID: PMC246832.

Patents

7,687,247 Process for controlling contamination of nucleic acid amplification reactions

7,670,823 Compositions for use in recombinational cloning of nucleic acids

7,455,974 Nucleic acid marker ladder for estimating mass

7,408,049 Recombinational cloning using nucleic acids having recombination sites

7,393,632 Use of multiple recombination sites with unique specificity in recombinational cloning

7,351,578 Use of multiple recombination sites with unique specificity in recombinational cloning

7,304,130 Recombinational cloning using engineered recombination sites

7,282,326 Recombinational cloning using engineered recombination sites

7,244,560 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

7,223,576 Recombinational cloning using engineered recombination sites

7,198,924 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites [Gateway Multisite]

7,132,520 Nucleic acid marker ladder for estimating mass

6,924,098 Nucleic acid ladders

6,720,140 Recombinational cloning using engineered recombination sites

6,680,378 Nucleic acid marker ladder for estimating mass

6,518,026 Process for controlling contamination of nucleic acid amplification reactions

6,287,823 Process for controlling contamination of nucleic acid amplification reactions

6,277,608 Recombinational cloning using nucleic acids having recombination sites

6,270,969 Recombinational cloning using engineered recombination sites

6,171,861 Recombinational cloning using engineered recombination sites

6,143,557 Recombination cloning using engineered recombination sites

5,970,782 Gradient filtration apparatus [Vertical membrane]

5,945,313 Process for controlling contamination of nucleic acid amplification reactions

5,888,732 Recombinational cloning using engineered recombination sites [Gateway cloning]

5,834,201 Nucleic acid marker ladder for estimating mass [Mass DNA ladder]

5,683,896 Process for controlling contamination of nucleic acid amplification reactions

5,449,758 Protein size marker ladder [Partial cyanogen bromide cleavage of a custom protein]

5,434,066 Modulation of CRE recombinase in the in vivo cloning of DNA [Based on plasmid incompatibilitiy]

5,106,727 Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers

5,043,272 Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers [An early version of whole genome amplification]

5,035,996 Process for controlling contamination of nucleic acid amplification reactions [UDG decontamination]

4,808,519 Method of detecting nucleic acid sequences [Based on transformation of E. coli]

4,403,036 Genetic reagents for generating plasmids containing multiple copies of DNA segments [Basis of 1 Kb DNA ladder]