User:James C. Schwabacher/Notebook/CHEM-571/2015/01/28

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective First trial of synthesizing PVA-Alginate beads with and without NaMT Procedure Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) Add alginate while stirring PVA-water solution ~0.1 g of nanocomposites should be added to this mixture where applicable Cool dissolved and mixed solution to 35°C Prepare 210 mL of saturated citric acid with 2% calcium chloride Add 4.2 grams of calcium chloride to 210 mL of water Add citric acid to saturation (32 g citric acid) 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a thin needle Stir for 24hrs to allow solidification Rinse with water to remove excess reactants Measurements PVA-Alg 1.015 g PVA 0.1649 g alginate PVA-Alg #2 0.998 g PVA 0.159 g Alg PVA-Alg-NaMT 0.999 g PVA 0.161 g alginate 0.109 g NaMT Saturated citric acid, 2% calcium chloride solution 4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride 32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid Notes PVA-Alg beads pumped with syringe made fiber like clusters not cooled at all dropped in the middle PVA-Alg-NaMT beads made with disposable dropper cooled 20 seconds in dropper dropped along side Turn heat off but keep stirring to prevent clumping pre-bead forming Let cool in dropper before dropping Move to Dr Fox's automated pumper when repeating bead synthesis Use wide dish PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping Don't fill disposable dropper all the way > cooling solution will come out Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.