Protocol
- Run fluorescence spectroscopy for the previously synthesized sets: Cys, GSH, 1-Thioglycerol.
- Finish precipitation of 80% BSA-CYS sample
- Prepare for the Sotelo-Gonzalez ("New") method with 80% BSA and Cysteine.
Description
The solutions created for tomorrow:
- 250mL of 20mM L-cysteine
- FW 121.16g/mol
- measure out: 0.6058g
- measured: 0.6063g
- actual conc: 19.98mM
- 50mL of 100mM ZnSO4
- FW 287.56g/mol
- measure out: 1.4378g
- measured: 1.4399 g
- actual conc: 100.1 mM
- 50mL of 10mM MnCl2
- FW 197.9g/mol
- measure out: 0.09895g
- measured: 0.0987g
- actual conc: 9.98 mM
- 10mL 1M NaOH
- FW 40.00g/mol
- 0.4284g
- Final concentration a bit over 1M check pH carefully when adjusting!
- 50mL 100mM Na2S
- FW 240.18g/mol
- measure out: 1.2009g
- measured: 1.2007g
- actual conc: 100 mM
Results
Notes
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