User:Jacob Esenther/Notebook/Chem 571/2014/10/22

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


  1. Ran Titration Analysis on KI-Colloid dialysis
  2. Ran Bradford Analysis on KI-Colloid dialysis


  1. Titration
    1. Added 3mL 1M HNO3 and 0.5mL KI solution to 10mL DI H2O
    2. Added 2 mL AgNO3 and swirl to complete precipitation
    3. Added 200 μL Ferric alum indicator
    4. Titrate with your NH4SCN solution
  2. Ran Bradford Analysis on KI-Colloid dialysis
    1. As expected, the Bradford analysis held a constant protein concentration
      • Lysozyme is too big to pass through the 3500 MWCO so it was constant on one side
      • KI is not expected to react with lysozyme so protein concentration was not affected



This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.