User:Jacob Esenther/Notebook/Chem 571/2014/08/27

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Biomaterials Design Lab Report.pngMain project page
Next entryResultset next.png


Preparation of Stock Solutions

Stock solutions of a protein (lysozyme), and HAuCl4 were prepared. The stock solutions were used to make six solutions of varying concentrations containing HAuCl4, lysozyme, and H20.


  1. Solutions of Colloidal Gold and Lysosyme were produced at different ratios to observe the formation of fibers
    1. Solutions were heated in a hot water bath and run through the Uv-vis to observe any color changes.
    2. Solutions were also cooked within the oven for an extended period of time to assist fiber formation.
  2. Kinetics of AuNP Formation
    1. Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution).
    2. The test tubes were covered with foil and placed in an 80°C hot water bath.
    3. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer.
    4. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.


Stock Solutions

  • HAuCl4 → 0.04621g / 50ml DI Water
  • Lysozyme → 0.0895g / 50ml DI Water

Gold - DI concentration Table.jpg


Using a teflon coated spatula is recommended so the HAuCl4 does not bind to the metal on the spatula.

During the heating process, the temperature fluctuated between 70-85°C