User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/27

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      

Stuff to make for M1:D1

  • finish cloning
    • 002, 003, 004 into no promotor plasmids
    • transform into E. coli.
  • overnight cultures
  • sterile water
  • aspirators need to be set up (for first day actually..)
  • add ethanol to PE
  • aliquot miniprep buffers (into 1.5mL centrifuge tubes)
    • P1 - 250uL
    • P2 - 250uL
    • N3 - 350uL
    • PE - 750uL
  • restriction enzymes... dilute before class? aliquot?