User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/08/25
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Jurkat E6-1 on VCAM-1
Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30 minutes at room temperature, and gently washed 2 times with Hepes buffer. Cells plated in growth media on the coverslips for 30 minutes at room temperature.
Cells develop nice uniformly thin lamella.