User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/07/07
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U937 Delta ST on VCAM-1
Cells from Alex on 07/01 (one week in growth media). Coverslips: base-treated as per Andrew's protocol, then coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30 minutes at room temperature, and gently washed 2 times with Hepes buffer. Plated cells resuspended in Hepes on the coverslips for 60 minutes at room temperature, then washed with Hepes two times to remove unattached cells.
Sample 1: Sells resuspended in Hepes on untreated coverslip. Cells are unattached and globular in shape.