User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/03/17

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U937 Delta ST FPR

Cells suspended in RPMI 1640, [math]1x10^7 cells/mL[/math]. Coverslips coated with Poly-L-lysine (P4832 SIGMA) for 30 minutes, washed 2 times with PBS. Plated media with cells for 30 minutes, then washed with PBS two times to remove suspended cells.

Sample 1: PBS, left at room temperature for 30 minutes

U937 01.jpg

Sample 2: N-fMLFF [math]10^{-7} \mu M[/math] in PBS, left at room temperature for 30 minutes

U937 02.jpg