# User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/02/19

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## Height calibration

Tangent average based on 10 measurements on the same 1 um bead, fit 0 to 80 sec: -0.0410

0.0410 pix/sec = 0.0410 pix/5nm = 0.0082 pix/nm. So 1 nm = 0.0082 pxl, or

### 1 pxl = 121.9 nm

Check: 40 steps 10 nm each, or 400 nm displacement

400 nm = 80*0.041 pxl; pxl = 5/0.041 nm = 121.9 nm

CHECK OK

Average SD: 0.061 pxl = 7.47 nm

### SD = 7.5 nm

2/22/2010 16:25

Linear Regression for A021910ZCAL09_E:

Y = A + B * X

Parameter Value Error

A 15.59389 0.00138

B -0.04374 3.00803E-5

R SD N P

-0.99819 0.0602 7668 <0.0001

2/22/2010 16:25

Linear Regression for A021910ZCAL10_E:

Y = A + B * X

Parameter Value Error

A 15.58053 0.00158

B -0.04197 3.45575E-5

R SD N P

-0.99741 0.06929 7677 <0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL08_E:

Y = A + B * X

Parameter Value Error

A 15.56832 0.00146

B -0.04236 2.72846E-5

R SD N P

-0.99829 0.06123 8289 <0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL07_E:

Y = A + B * X

Parameter Value Error

A 15.18403 0.00152

B -0.0398 3.25563E-5

R SD N P

-0.99739 0.06706 7818 <0.0001

2/22/2010 16:26

Linear Regression for A021910ZCAL06_E:

Y = A + B * X

Parameter Value Error

A 15.14745 0.00136

B -0.04008 2.91786E-5

R SD N P

-0.99794 0.06002 7803 <0.0001

2/22/2010 16:27

Linear Regression for A021910ZCAL05_E:

Y = A + B * X

Parameter Value Error

A 15.8854 0.00847

B -0.04232 1.76769E-4

R SD N P

-0.94234 0.32369 7230 <0.0001

2/22/2010 16:27

Linear Regression for A021910ZCAL04_E:

Y = A + B * X

Parameter Value Error

A 15.31804 0.00147

B -0.04216 3.19662E-5

R SD N P

-0.9978 0.06434 7691 <0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL03_E:

Y = A + B * X

Parameter Value Error

A 15.54347 0.00142

B -0.04112 3.05693E-5

R SD N P

-0.99785 0.06271 7797 <0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL02_E:

Y = A + B * X

Parameter Value Error

A 15.61833 0.00138

B -0.04034 3.02747E-5

R SD N P

-0.99785 0.06036 7668 <0.0001

2/22/2010 16:28

Linear Regression for A021910ZCAL01_E:

Y = A + B * X

Parameter Value Error

A 15.28446 0.00179

B -0.03691 3.91125E-5

R SD N P

-0.99571 0.07814 7683 <0.0001