User:Howard Boland/Notebook/Art from Synthetic Biology/2011/03/08

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Check Transformation 1>12O, XL-1 BLUE, ok 1>12O, XL-1 BLUE, ok 1>12O, DH5-a, blank 1>22I, DH5-a, blank 1>22I, XL-1 BLUE, blank Overnight Growth 1>12O, E0840, Ok (1.5OD) -> Stock + Moved 1ml into fresh broth and continue growing 1>22I, I13504, Ok (0.9OD) -> Stock + Moved 1ml into fresh broth and continue growing 1>6I, E0040, appears dark (possible smudging of from plastic in bottle) -> Stock + Moved 1ml into fresh broth and continue growing Miniprep 5ml 1>12O, E0840 -> Elute in 35µl H20 5ml 1>22I, I13504 -> Elute in 35µl H20 Gel D.D. R0040 10µl 1kb Ladder BLANK 30µl R0040 E/S (H.B.) BLANK 30µl R0040 E/S (A.A.) BLANK 5µl R0040 E/S (H.B.) 5µl R0040 E/S (A.A.) Gel Plasmids 5µl 1kb Ladder BLANK BLANK 5µl 12O BLANK 5µl 22I BLANK 10µl R0040 (0.5OD>5ml>A.A)