User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/21
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Check TransformationExpecting zero colonies for pSB1A3 E|P and multiple colonies from BBa_J23100 Miniprep overnight growthDigestionBBa_I13504 will be a downstream part, and thus digested with XbaI and PstI Gel DigestionLigation
Incubate for 2 hours in room temperature TransformationLigated part Overnight growthBBa_J23100 (test promoter)
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