User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/21

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Check Transformation

Expecting zero colonies for pSB1A3 E|P and multiple colonies from BBa_J23100

Miniprep overnight growth

Digestion

BBa_I13504 will be a downstream part, and thus digested with XbaI and PstI

Gel Digestion

Ligation

  1. 7ul BBa_I13504 X|P
  2. 7ul BBa_katE E|S
  3. 1ul pSB1A3 E|P

Incubate for 2 hours in room temperature

Transformation

Ligated part

Overnight growth

BBa_J23100 (test promoter)