User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/01
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Check Transformation of BBa_I13504
Around 10 colonies where produced.
Preparation of M9 Media for YYC912
1M MgSO4, 10ml
MgSO4x7H20 has a molecular weight of FW 246.48.
1M CaCl2, 10ml
CaClx6H2O has a molecular weight of FW 219.08.
10% Casamino Acids
1g/l to make 10% Stock Casamino Acids 0.1g to make 100ml 10% Stock Casamino Acids
Setup overnight growth of BBa_I13504 colonies from plate
I prepared 3 bottles with ampicillin and inoculated with colonies from the transformation