User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/18
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Digestion pSense66 PstI and XhoI/BamHI
For each of two reaction tubes add 48µl Master Mix
Incubate in 37ºC for 2 hours
I set up a gel to check the miniprep and the digestion results.
I put on 4 bottles for overnight growth using glycerol stock.