User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/15
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PCR Product, 2.3kb product
I ran a repeat of previous PCR from 4th of November 2010.
Aliquote into each of 2 tubes
Cycles: 30x Lid: 100ºC Volume: 50µl (each)
I prepared a 1% Agarose Gel
Purification of Gel products
After cutting the product, I purified it together with last weeks digested PCR product.
At this point I am interested in whether or not the 2300bp products can be broken into fragments. According to the in sillico this should not be possible but the result of last weeks digestion indicated that this was possible. If my product remains at 2300bp then I am pretty certain that this is my product.
Total mix is 60µl ensure it is well mixed.
For each of 3 tubes add
Mix well and place in 37ºC for 2 hours
Gel Result of digested 2300bp PCR product
I am expecting the result of the digestion to resolve as one band only and additional faint band represents possible undigested bits. I ran the digestion on a 1% gel.