User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/10

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Result of overnight growth

The result of the overnight growth was positive but took time to manifest due to someone having changed the temperature in the shaker.

Glycerol Stock

I took glycerol stock of the colony before proceeding with the miniprep

  1. 700µl stock
  2. 300µl 50% glycerol

store at -80ºC

Miniprep

I then proceeded by minipreping the colony using the QIAgen kit to obtain 30µl plasmid.