User:Howard Boland/Notebook/Art from Synthetic Biology/2010/10/31
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I check the plate with the transformed cells with yesterdays ligation between the digested PCR products pSense66(2.3kb) and pBR322(790kb). All sites are PstI digested and purified. I had no growth on the plate.
PCR Gel result of 2.3kb product
I ran out yesterdays PCR product on a 1% gel to see if I had any new result using the same conditions as in the beginning of the week. In addition I also ran out the ligation samples and yesterdays miniprep.
Error: Again I have no result from the PCR reaction!!!