User:Howard Boland/Notebook/Art from Synthetic Biology/2010/10/31
From OpenWetWare
Project name | Main project page Previous entry Next entry |
Check TransformationI check the plate with the transformed cells with yesterdays ligation between the digested PCR products pSense66(2.3kb) and pBR322(790kb). All sites are PstI digested and purified. I had no growth on the plate. PCR Gel result of 2.3kb productI ran out yesterdays PCR product on a 1% gel to see if I had any new result using the same conditions as in the beginning of the week. In addition I also ran out the ligation samples and yesterdays miniprep.
Error: Again I have no result from the PCR reaction!!! |