User:Howard Boland/Notebook/Art from Synthetic Biology/2010/10/31

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Check Transformation

I check the plate with the transformed cells with yesterdays ligation between the digested PCR products pSense66(2.3kb) and pBR322(790kb). All sites are PstI digested and purified. I had no growth on the plate.

PCR Gel result of 2.3kb product

I ran out yesterdays PCR product on a 1% gel to see if I had any new result using the same conditions as in the beginning of the week. In addition I also ran out the ligation samples and yesterdays miniprep.

  1. Lane 1: 10µl 1kb NEB Quick Ladder
  2. Lane 2: 50µl Pcr product, expected 2.3kb (template pUA66katE)
  3. Lane 3: 50µl Pcr product, expected 2.3kb (template pUA66katE)
  4. Lane 4: 5µl pSense66 (pcr product digested and purified from earlier in the week)
  5. Lane 5: 5µl pBR322 (pcr product digested and purified from earlier in the week)
  6. Lane 6: 5µl pBR322 (pcr product digested and purified from earlier in the week)
  7. Lane 7: 5µl pUA66katE (gel check of yesterdays miniprep)
  8. Lane 8: 5µl pUA66katE (gel check of yesterdays miniprep)

31112010-pcr-andother.jpg

Error: Again I have no result from the PCR reaction!!!