User:Howard Boland/Notebook/Art from Synthetic Biology/2010/09/10

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Overnight Growth Result All 6 samples grew well. To ensure I had sufficient original stock, I made 3 tubes of glycerol stock of pUA66 and stored at -80ºC. Streaking Result Both plates grew. However, none of the colonies on the pUA66katE#2, Blue exhibited visible expression of GFP. Glycerol stock: 700µl pUA66 300µl 50% Glycerol The remaining 5 samples were prepared for miniprep as I already had stock of these Miniprep Digestion I digested 3 samples of different stock using 15µl of DNA. Mastermix: ---to come Gel Result I ran both the 5 miniprep samples (2µl each) and the 3 digested samples on two different gels. The Miniprep samples were run on a 1% gel to check for genomic contamination - none where found but the position of the bands surprised me. 5µl 1kb NEB Quick Ladder 2µl pUA66katE#2, Blue 2µl pUA66katE#2, Blue 2µl pUA66katE#2, Green 2µl pUA66katE#2, Green 2µl pUA66katE#2, Black The 3 digested samples where run on a 2% gel with a 100bp ladder. 5µl 100bp NEB Ladder 30µl pUA66katE#2, Blue 30µl pUA66katE#2, Green 30µl pUA66katE#2, Black Fluorescent Microscopy outcomes Some background flourescent was observed