User:Howard Boland/Notebook/Art from Synthetic Biology/2010/08/24
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Check overnight growth
I checked overnight growth of nine 10ml bottles with kanamycin and selected colonies.
My question was - whether the SAP treatment ensured the presence of my insert given that the colonies grew?
I proceeded using 2 bottles of pUA66katE#A1#2 for miniprep, I kept one to preform assay and the last bottle was centrifuged and the cell pellets frozen. For the SAP treated vectors, 2 were used for miniprep. For the standard ligation 1 bottle was used for miniprep. All remaining bottles were centrifuged and the cell pellets frozen at -20ºC.
I decided to do a miniprep on 4 of 8 bottles:
In order to preserve any sample that screened positive for the insert, I prepared glycerol stock.
For each of the 5 samples: