User:Howard Boland/Notebook/Art from Synthetic Biology/2010/08/21
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All but the p67 ligation grew. Why the p67 didn't grow is possible due to a short ligation period. The SAP treated vectors also seemed to ligase - I am quite curious if these ligations are all with the insert. If they are not I would suspect this is due to having the buffer condition wrong. I should also have done a self-ligating SAP test using T4.