User:HolyRomulus/Notebook/Female Growth and Development Study Telomere Length Assays/2018/09/11

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September 11, 2018

FGDS Telomere Assay Troubleshooting Pt. 1

Today I am running am mini-plate to test my telomere primers. Specifically, I am running 25 samples using a mastermix generated using my primer working stock (Stock A) and 25 samples using a mastermix comprised of a fresh 10uM working dilution of the reverse and forward telomere primers. The fresh dilution was made using the same 100uM factory stock that I used for Stock A. My concern is that the diluted primers have degraded over time due to storage at 4C.

Results

Nothing amplified. Either my Sybergreen is bad or the factory stock has also degraded in a manner similar to the dilution.