from grown cultures, made 1mL MP into 30UL. Digested 335, 832, A, B, C via XP 1h50.
(Expected 3450... 335 expected is 2900...)
ambig. results. respiked 2uL colonies of ABC, C, B+C, A+C at 9PM sharp. Made frozen stock of ABC called "J36937 col 'c' 12.15" on yellow sticker in -80 (1mL cells, spin down, add 1mL LB=20%glyc. Redid digest of 832 and col 'c', This time in 20uL and with SP cut. 12 H digest, when running on gel run for awhile.