hetmann did pcr test of 4 colonies. running on egel.
grew samples up. MP and digesting.
after pcr result
i suspect we have what we want, but the polyermase cannot go 4kb in length (which is pretty damn long). grew up A B C D in 2uLLBKAN. also grew up, starting 1130PM, A+C, B+C, C, from stock AND ABC (A) ABC (B) ABC (C) ABC (D) from 1uL of the liquid culture started at 830. This will be used, if the digest tomorrow is correct, for the oscillation and interaction exp.